Extraction and Purification of Pseudomonas aeruginosa Lipopolysaccharide Isolated from Wound Infection

Authors

  • Israa M. Subhi Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author
    • ayaid K. Zgair Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author
      • Jenan A. Ghafil Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author

        Keywords:

        Gel filtration chromatography, hotEDTA, Lipopolysaccharide, Pseudomonas aeruginosa, Sephadex G-200, VITEK 2 system.

        Abstract

        Samples were obtained from patients suffering from burns and wounds and then identified using the biochemical tests and the VITEK 2 fluorescent system. The tests confirmed the presence of Pseudomonas aeruginosa. Antibiotic susceptibility testing showed that the isolate was sensitive to piperacillin, ceftazidime, cefepime, imipenem, meropenem, amikacin, gentamicin, tobramycin, ciprofloxacin and levofloxacin while resistant to ampicillin, cefazolin, ceftriaxone, tigecycline and trimethoprim/sulfamethoxazole. The lipopolysaccharide (LPS) was extracted by the hotEDTA method and partially purified by gel filtration chromatography using the Sephadex G-200.

         

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        Published

        2017-01-12

        Issue

        Volume 5, Number 1 (2017)

        Section

        Research article

        License

        Copyright (c) 2017 Authors

        Creative Commons License

        This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

        All articles in the World Journal of Experimental Biosciences are published under the terms of the Creative Commons  Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

        How to Cite

        [1]
        Israa M. Subhi et al. trans. 2017. Extraction and Purification of Pseudomonas aeruginosa Lipopolysaccharide Isolated from Wound Infection. World Journal of Experimental Biosciences. 5, 1 (Jan. 2017), 5–8.

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