The effect of Nd: YAG lasers on Leishmania donovani promastigotes

Authors

  • Hanna S. Sabaa Department of Physics, College of Science, Al- Mustansiriya University, Baghdad, Iraq. Author
    • Khawla H. Zghair Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author
      • Nebras R. Mohammed Department of Biology, College of Science, Al- Mustansiriya University, Baghdad, Iraq. Author
        • Israa S. Musa Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq Author
          • Raad S. Abd Department of Physics, College of Science, Al- Mustansiriya University, Baghdad, Iraq. Author

            Keywords:

            Attenuated promastigotes, Leishmania donovani, Nd: YAG laser

            Abstract

            The present study was conducted to assess the effect of Nd:YAG laser on Leishmania donovani promastigotes. L. donovani promastigotes exposed to Nd:YAG laser (wavelength 1060 A°) in 500, 1000 and 1500 pulse between each pulse and another 6 seconds in comparison to the control (L. donovani promastigotes without exposure to Nd:YAG laser. The effect of Nd:YAG laser on the viability of L. donovani promastigotes was measured using the colorimetric MTT assay and the percentage of viability was counted. L. donovani viable cells were decreased with long exposure to Nd-YAG laser. Nd:YAG laser was effective in killing most of L. donovani promastigotes, and the remaining were devoid of flagellum that may lost their ability to cause infection.

             

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            Published

            2016-01-10

            Issue

            Volume 4, Number 1 (2016)

            Section

            Research article

            License

            Creative Commons License

            This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

            All articles in the World Journal of Experimental Biosciences are published under the terms of the Creative Commons  Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

            How to Cite

            [1]
            Hanna S. Sabaa et al. trans. 2016. The effect of Nd: YAG lasers on Leishmania donovani promastigotes. World Journal of Experimental Biosciences. 4, 1 (Jan. 2016), 25–28.

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