Isolation and identification of Cronobacter spp. obtained from different food samples in Iraq

Authors

  • Alyaa Razooqi AL-Lami AL-Lami Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author
    • Sanaa Burhan Abd -Aljalil Author
      • Hula Younis Fadel Author

        Keywords:

        Antibiotic susceptibility, Biochemical test, Cronobacter spp, food samples, ITS regions, PCR

        Abstract

        The study aims to isolate and identify Cronobacter spp isolated from different food in Iraq. The identification of Cronobacter depended on biochemical profile, VITEK2 system and polymerase chain reaction (PCR) technique. The antibiotic susceptibility for six recovered isolates was done also. 120 food samples were tested. Cronobacter spp was identified in 4 (3.33%) food samples by VITEK2 system. The internal transcribed spacer (ITS) regions were detected in 11 (9.16%) isolates of Cronobacter spp. Six isolates of Cronobacter spp were tested for antibiotic susceptibility (8 antibiotics) using disc diffusion method. The results showed that 5 isolates (83.3%) were resistant to ampicillin and cephalothin, and 2 isolates (33.3%) were resistant to kanamycin, streptomycin and tetracycline. While, 5 isolates (83.3%) were sensitive to chloramphenicol, 4 isolates (66.7%) were sensitive to tetracycline, and 3 isolates (50%) were sensitive to gentamicin and nalidixic acid.

         

        Downloads

        Published

        2015-09-06

        Issue

        Volume 3, Number 2 (2015)

        Section

        Research article

        License

        Copyright (c) 2015 Authors

        Creative Commons License

        This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

        All articles in the World Journal of Experimental Biosciences are published under the terms of the Creative Commons  Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

        How to Cite

        [1]
        AL-Lami, A.R.A.-L. et al. trans. 2015. Isolation and identification of Cronobacter spp. obtained from different food samples in Iraq. World Journal of Experimental Biosciences. 3, 2 (Sep. 2015), 69–73.