Effect of cultural conditions on lipase production Pseudomonas aeruginosa isolated from Iraqi soil

Authors

  • Jenan Atiyah Ghafil Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author
    • Shatha Salman Hassan Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author

      Keywords:

      Pseudomonas, glucose, urea, lipase, crude oil, tween 20

      Abstract

      Seventy Pseudomonas isolates were obtained from different Iraqi soils samples. Thirty five of these isolates have the ability to produce lipase. These isolates were screened on Rhan medium. The highest lipase activity was found with P. aeruginosa. ps12. The effect of media composition, pH and temperature on production of lipase were determined. Maximum lipase production appeared in the medium containing (0.5%) glucose as carbon source, (0.5%) urea as nitrogen source and 1% of olive oil with pH 8 and incubated at 30 OC for 48 h with 1 ml (O.D 600= 0.8) of 24 h of bacterial culture.

       

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      Published

      2014-05-10

      Issue

      Volume 2, Number 1, (2014)

      Section

      Research article

      License

      Creative Commons License

      This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

      All articles in the World Journal of Experimental Biosciences are published under the terms of the Creative Commons  Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

      How to Cite

      [1]
      Jenan Atiyah Ghafil and Shatha Salman Hassan trans. 2014. Effect of cultural conditions on lipase production Pseudomonas aeruginosa isolated from Iraqi soil. World Journal of Experimental Biosciences. 2, 1 (May 2014), 13–18.

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