Bacteriocin Produced by Lactobacillus acidophilus Exhibits Antagonistic Activity Against Uropathogenic Klebsiella pneumoniae

Authors

  • Jenan Atiyah Ghafil Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Author
    • Zainab Hani Hatif Scientific Research Commission, Ministry of Higher Education and Scientific Research, Baghdad, Iraq. Author

      DOI:

      https://doi.org/10.65329/wjeb.v13.02.004

      Keywords:

      Antibiofilm, Antagonism, Bacteriocin, Klebsiella pneumoniae, UTI.

      Abstract

      Antibiotic resistance in bacteria is a major problem facing the health system worldwide. The ability of bacteria to form biofilms enhances their resistance to antibiotics. Finding alternative antimicrobial agents is a crucial approach in the global fight against infectious diseases caused by multidrug-resistant bacteria. In this study, yogurt samples were cultured on Man–Rogosa–Sharpe agar (MRS) to isolate Lactobacillus acidophilus. Fifty urine samples were collected from patients with urinary tract infections (UTI). Eleven isolates of Klebsiella pneumoniae were obtained from these samples. The bacteriocin was extracted from L. acidophilus grown in MRS broth. Ammonium sulfate at 70% saturation was used for the partial purification of the bacteriocin. The gel diffusion method evaluated the antibacterial effect of both crude and partially purified bacteriocin on MDR-K. pneumoniae, which produced the highest level of biofilm. The ability of the 11 isolates of K. pneumoniae to produce biofilm was assessed using the microdilution method in a polystyrene microtiter plate. The results showed that out of eleven isolates, eight were MDR. All isolates produced biofilm at different levels. Both crude and partially purified bacteriocin (at dilutions 1/2, 1/4, 1/8, 1/16, and 1/32) inhibited the growth of Kp9. The partially purified bacteriocin was more effective than the crude form in inhibiting Kp9 growth. Sub-MICs (1/2, 1/4, 1/8, and 1/16 MICs) of both crude and partially purified bacteriocin inhibited biofilm formation of Kp9. The bacteriocin from L. acidophilus has the ability to inhibit MDR-K. pneumoniae and its capacity to prevent biofilm formation in vitro.  

      Author Biographies

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      Published

      2025-12-17

      Issue

      Volume 13, Number 2 ( July 2025) (5 articles)

      Section

      Research article

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      All articles in the World Journal of Experimental Biosciences are published under the terms of the Creative Commons  Attribution 4.0 International License (CC BY 4.0) ( (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

      How to Cite

      [1]
      Jenan Atiyah Ghafil and Zainab Hani Hatif trans. 2025. Bacteriocin Produced by Lactobacillus acidophilus Exhibits Antagonistic Activity Against Uropathogenic Klebsiella pneumoniae. World Journal of Experimental Biosciences. 13, 2 (Dec. 2025), 037 – 041. DOI:https://doi.org/10.65329/wjeb.v13.02.004.

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